期刊
JOURNAL OF PHYSICAL CHEMISTRY C
卷 115, 期 11, 页码 4837-4842出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp1083482
关键词
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资金
- National Basic Research Program of China [2010CB732403]
- NSFC [50503006, 20735002, 40940026]
- Science foundation of Fujian Province of China [2008J0149, JA10017]
Fluorescence spectra of thymine-containing oligonucleotides labeled with donor/acceptor in the presence of Hg2+ ions were investigated. Formation of T-Hg2+-T base pairs gives rise to a hairpin structure and makes both termini of the oligonucleotide close to each other. For fluorescein or tetramethylrhodarrine single-labeled oligonucleotide, fluorescence quenching was observed on addition of Hg2+ ions. For fluorescein and tetramethylrhodamine double-labeled oligonucleotide, the apparent FRET efficiencies decrease unexpectedly in the presence of Hg2+ ions. The unusual fluorescence quenching in the presence of Hg2+ ions was ascribed to formation of T-Hg2+-T base pairs, which can accept and mediate the electron transfer and provide an additional de-excitation process for the excited state of fluorophores via photoinduced electron transfer.
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