期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 118, 期 28, 页码 8313-8329出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp501778z
关键词
-
资金
- National Institute of General Medical Sciences Grant [R01GM085437]
This article surveys the recent application of optical Fourier processing to the long-established but still expanding field of single-molecule imaging and microscopy. A variety of single-molecule studies can benefit from the additional image information that can be obtained by modulating the Fourier, or pupil, plane of a widefield microscope. After briefly reviewing several current applications, we present a comprehensive and computationally efficient theoretical model for simulating single-molecule fluorescence as it propagates through an imaging system. Furthermore, we describe how phase/amplitude-modulating optics inserted in the imaging pathway may be modeled, especially at the Fourier plane. Finally, we discuss selected recent applications of Fourier processing methods to measure the orientation, depth, and rotational mobility of single fluorescent molecules.
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