期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 117, 期 5, 页码 1321-1325出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp4001187
关键词
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资金
- KAKENHI [23550019]
- Mitsubishi Foundation
- Grants-in-Aid for Scientific Research [23510251, 22590049, 23550019] Funding Source: KAKEN
We have developed a near-infrared excited Raman optical activity (ROA) spectrometer and report the first measurement of near-infrared ROA spectra of a light-driven proton pump, bacteriorhodopsin. Our results demonstrate that a near-infrared excitation enables us to measure the ROA spectra of the chromophore within a protein environment. Furthermore, the ROA spectra of the all-trans, 15-anti and 13-cis, 15-syn isomers differ significantly, indicating a high structural sensitivity of the ROA spectra. We therefore expect that future applications of the near-infrared ROA will allow the experimental elucidation of the active site structures in other proteins as well as reaction intermediates.
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