期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 115, 期 10, 页码 2417-2423出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp109661c
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资金
- Slovak Ministry of Education [1/0124/08, 3/5115/07, LPP-0290-09, APVV-0449-07]
- Agency of the Ministry of Education of the Slovak Republic [034/2009/2.1/OPRD, 26220120024]
Hypericin (Hyp) is a natural photosensitizing pigment with a possible application in the photodynamic therapy of cancer. Hyp is readily dissolved in dimethylsulfoxide (DMSO) but forms nonsoluble aggregates in an aqueous environment. Fluorescence spectroscopy and diffusion coefficient measurements are used to investigate the self-association of Hyp molecules in DMSO/water mixtures. Fluorescence measurements reveal that Hyp remains in its monomeric form in DMSO/water mixtures containing up to similar to 20-30 wt % water. At higher water concentration, Hyp starts to form nonfluorescent aggregates. To determine the size of the aggregates, the diffusion coefficient of Hyp is determined for different DMSO/water mixtures both experimentally and theoretically. Our data indicate that the size of the aggregates increases as more water is added into DMSO. At SO wt % water content, the effective diffusion coefficient is about 30% smaller than the calculated value for the stacked Hyp tetramer. The results indicate that in an aqueous environment, Hyp presumably produces large molecular weight stacked H-aggregates. We have also confirmed that in an aqueous environment at alkaline pH, molecules of Hyp remain in the monomeric state.
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