期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 114, 期 45, 页码 14373-14382出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp100353v
关键词
-
资金
- NIH USA [HL081273, EB007279]
- NIH [P41 RR001348]
Oxygen concentration distributions in biological systems can be imaged by the phosphorescence quenching method in combination with two-photon laser scanning microscopy In this paper, we identified the excitation regime in which the signal of a two-photon-enhanced phosphorescent probe (Finikova O S, Lebedev A Y, Aprelev, A, Troxler, T, Gao, F, Garnacho, C, Muro, S Hochstrasser, R M, Vinogradov, S A ChemPhysChem 2008 9, 1673-1679) is dependent quadratically on the excitation power (quadratic regime), and performed simulations that relate the photophysical properties of the probe to the imaging resolution Further we characterized polymersomes as a method of probe encapsulation and delivery Photophysical and oxygen sensing properties of the probe were found unchanged when the probe is encapsulated in polymersomes Polymersomes were found capable of sustaining high probe concentrations, thereby serving to improve the signal-to-noise ratios for oxygen detection compared to the previously employed probe delivery methods Imaging of polymersomes loaded with the probe was used as a test-bed for a new method
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据