期刊
JOURNAL OF PHYCOLOGY
卷 47, 期 3, 页码 591-602出版社
WILEY
DOI: 10.1111/j.1529-8817.2011.00980.x
关键词
actin microfilaments; cell wall; desiccation; DIOC6; FM 1-43; green algae; Streptophyta; ultrastructure
资金
- Alpine Research Center Obergurgl
- Deutsche Forschungsgemeinschaft (DEG) [KA899/16-1/2]
Klebsormidium crenulatum (Kutz.) Lokhorst (Klebsormidiophyceae, Streptophyta) isolated from an alpine soil in Tyrol, Austria, was experimentally exposed to desiccation under various relative air humidities (RH 5, 75, and >95%, ambient air 55%-60%). The effects on the structure and ultrastructure of K crenulatum after 1, 4, or 7 d of desiccation at 5, 75, and >95% RH were investigated. The cross walls were deformed to an undulated shape, and the cell diameter was reduced to similar to 60% of the control. Regardless of the RH applied, in all cases the cytoplasm appeared denser compared to that of liquid-culture-grown cells. Electron-dense particles with diameters of 0.4 mu m-0.8 pm were observed in the cytoplasm, likely representing lipid droplets. The chloroplasts of desiccated samples contained a large number of plastoglobules. The number and appearance of mitochondria were not visibly altered, as also verified by 3,3' dihexyloxacarbocyanine iodine (DIOC6) staining. The amphi-philic styryl dye FM 1-43 resulted in staining of the plasma membrane in cells from liquid culture. In 7 d desiccated samples, a marked fluorescence is seen in similar to 40%-50% of the cells, which were dead. Actin microfilaments (MFs) were drastically disrupted after desiccation; only dotlike actin batches remained. These results demonstrate that flexibility of the cell walls and maintenance of the key organelles play a key role in the tolerance of desiccation stress in K crenulatum.
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