期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 187, 期 -, 页码 41-47出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2018.07.032
关键词
Diabetes mellitus; Photobiomodulation therapy; Laser versus LED; Diabetic wound healing; Mitochondrial fission and fusion; Collagen fibers; Atomic force microscopy
资金
- Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico - FUNCAP [0778929/2015, 99999.006648/2015-00]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior Capes
- US NIH [R01AI050875, R21AI121700]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI050875, R21AI121700] Funding Source: NIH RePORTER
Objective: Mitochondrial dysfunction has been associated with the development of diabetes mellitus which is characterized by disorders of collagen production and impaired wound healing. This study analyzed the effects of photobiomodulation (PBM) mediated by laser and light-emitting diode (LED) on the production and organization of collagen fibers in an excisional wound in an animal model of diabetes, and the correlation with inflammation and mitochondrial dynamics. Methods: Twenty Wistar rats were randomized into 4 groups of 5 animals. Groups: (SHAM) a control nondiabetic wounded group with no treatment; (DC) a diabetic wounded group with no treatment; (DLASER) a diabetic wounded group irradiated by 904 nm pulsed laser (40 mW, 9500 Hz, 1 min, 2.4 J); (DLED) a diabetic wounded group irradiated by continuous wave LED 850 nm (48 mW, 22 s, 1.0 J). Diabetes was induced by injection with streptozotocin (70 mg/kg). PBM was carried out daily for 5 days followed by sacrifice and tissue removal. Results: Collagen fibers in diabetic wounded skin were increased by DLASER but not by DLED. Both groups showed increased blood vessels by atomic force microscopy. Vascular endothelial growth factor (VEGF) was higher and cyclooxygenase (COX2) was lower in the DLED group. Mitochondrial fusion was higher and mitochondrial fusion was lower in DLED compared to DLASER. Conclusion: Differences observed between DLASER and DLED may be due to the pulsed laser and CW LED, and to the higher dose of laser. Regulation of mitochondrial homeostasis may be an important mechanism for PBM effects in diabetes.
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