期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 141, 期 -, 页码 47-58出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2014.09.003
关键词
Spectroscopic titrations; DNA-binding; Intercalative; Docking; Comet assay; Phosphate backbone
资金
- OU-DST PURSE [A-30]
- CFRD Osmania University, Hyderabad
Three new ruthenium(II) polypyridyl complexes [Ru(phen)(2)BrIPC](2+) (1), [Ru(bpy)(2) BrIPC](2+) (2) and [Ru(dmb)(2)BrIPC](2+) (3) where, BrIPC = (6-bromo-3-(1H-imidazo[4,5-f] [1,10]-phenanthroline, phen = 1,10-phenanthroline, bpy = 2,2' bipyridine, dmb = 4,4'-dimethyl 2,2' bipyridine, were synthesised and characterised. DNA-binding nature was investigated by spectroscopic titrations and mode of binding was assessed by viscosity measurements. The DNA-binding constants K-b of complexes 1, 2 and 3 were determined to be in the order of 10(5). Experimental results showed that these complexes interact with CT-DNA by intercalative mode. Photocleavage and antimicrobial activities were complex concentration dependent, at high concentration, high activity and vice versa. MTT assay was performed on HeLa cell lines, IC50 values of complexes in the order of 3 > 2 > 1 > cisplatin. From comet assay, cellular uptake studies, we observed that complexes could enter into the cell membrane and accumulate inside the nucleus. Molecular docking studies support the DNA binding affinity with hydrogen bonding and van der Waals attractions between base pairs and phosphate backbone of DNA with metal complexes. (C) 2014 Elsevier B.V. All rights reserved.
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