期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 128, 期 -, 页码 35-42出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2013.08.006
关键词
Proteins; Inhibition; Spectroscopy; Isothermal titration calorimetry; Thermodynamics; Molecular docking
资金
- NSFC [21277081]
- Key Scientific and Technical Innovation Project, Ministry of Education of China [708058]
- Independent innovation program of Jinan [201202083]
- Independent innovation foundation of Shandong University natural science projects [2012DX002]
Chrysoidine is an industrial azo dye and the presence of chrysoidine in water and food has become an environmental concern due to its negative effects on human beings. In this work, the interactions between chrysoidine and bovine liver catalase (BLC) were explored. Obvious loss in catalytic activity was observed after incubation of BLC with chrysoidine, and the inhibition effect of BLC was found to be of the non-competitive type. No profound conformational change of BLC occurs in the presence of chrysoidine as revealed by UV-vis absorption, circular dichroism and fluorescence spectroscopy studies. Isothermal titration calorimetry results indicate that catalase has two sets of binding sites for chrysoidine. Further, molecular docking simulations show that chrysoidine is located within the bottleneck in the main channel of the substrate to the active site of BLC, which explain the activity inhibition of BLC by chrysoidine. (C) 2013 Elsevier B.V. All rights reserved.
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