期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 126, 期 -, 页码 1-10出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2013.06.005
关键词
Nile red; DMPG; DMPC; Lipids; beta-Cyclodextrin; Fluorescence
资金
- Department of Science and Technology, Government of India
- C.S.I.R.
- U.G.C.
Fluorometric measurements are exploited to explore the binding interactions of nile red (NR) with anionic lipid dimyristoyl-L-alpha-phosphatidylglycerol (DMPG), zwitterionic lipid dimyristoyl-L-alpha-phosphatidyl-choline (DMPC) as well as neutral cyclic oligosaccharide beta-cyclodextrin (beta-CD) solutions. The binding constants are found to be quite high and comparable (within a factor of five). Series of spectral techniques like steady state fluorescence and fluorescence anisotropy study, micropolarity study, quenching study and time resolved experiments reveal that the addition of beta-CD to the probe-lipid complexes results in weakening of the lipid-probe interaction and formation of probe-beta-CD inclusion complexes leading to the removal of some of the probe (NR) molecules from the lipid environments. The extent of removal of NR is, however, more from DMPG than DMPC lipid. The phenomena are explained from the concept of competitive binding of the probe between the lipids and beta-CD. Since lipids are the principal constituents of the cell walls, the work might make a foundation for the possible removal of excess of molecules like nile red adsorbed on the cell walls. (c) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据