期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 115, 期 -, 页码 63-72出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2012.06.012
关键词
CT-DNA binding; 5 '-GMP; DNA cleavage; Topo-I inhibition; Antitumor activity; Molecular docking
资金
- UGC, New Delhi [F31-100/2005(SR)]
The new heterobimetallic Cu-II-Sni(2)(IV)/Ni-II-Sn-2(IV) complexes 1 and 2 bearing bioactive pharmacophore ligand scaffold; 1,10-phenanthroline and ethylenediamine were synthesized and characterized by spectroscopic (IR, UV-vis, NMR, ESI-MS) and analytical methods. The in vitro DNA binding studies of 1 and 2 with CT-DNA were carried out by employing various biophysical methods which reveal strong electrostatic binding via phosphate backbone of DNA helix, in addition to partial intercalation in the minor groove and stabilized by intramolecular hydrogen bonding. To gain further insight into the molecular recognition at the target site, UV-vis titrations of 1 with 5'-GMP was carried out and validated by H-1 and P-31 NMR. Complex 1 cleaved pBR322 DNA via oxidative pathway and exhibited high inhibition activity against Topo-I at 20 mu M. Furthermore, the cytotoxicity of 1 was examined on a panel of human tumor cell lines of different histological origins showing promising antitumor activity. (C) 2012 Elsevier B.V. All rights reserved.
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