期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 103, 期 2, 页码 166-179出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2011.03.001
关键词
Modulated Schiff base; Cu(II)/Zn(II) complexes; In vitro DNA binding studies; Nucleotides interaction; Cleavage activity
资金
- Department of Biotechnology, New Delhi [BT/PR9208/Med/30/13/2007]
New Schiff base ligand L derived from the condensation reaction of 2-amino-3-formylchromone with (R)-2-amino-2-phenylethanol was synthesized and characterized which involves combination element of ammine functionality and naturally occurring heterocyclic chromone, 4H-benzopyran-4-one. Subsequently, their complexes 1 and 2 with Cu(NO3)(2) and Zn(NO3)(2), respectively were prepared. The DNA binding studies of the ligand L and complexes 1 and 2 with CT-DNA as compared to classical anticancer drug cisplatin were carried out by employing different optical methods viz, UV-vis, fluorescence, circular dichroism and viscosity measurements. Furthermore, the absorption studies, H-1 and P-31 with mononucleotides were also monitored to examine the base specific interactions of the transition metal complexes which revealed a higher propensity of copper(II) complex 1 for 5'-GMP while for zinc(II) complex 2 towards 5'-TMP involving groove binding mechanism of the complexes towards DNA. The complex 1 exhibits a remarkable DNA cleavage activity with pBR322 DNA in presence of different activators and cleavage reaction involves various oxygen species suggesting the involvement of active oxygen species for the DNA scission. (C) 2011 Elsevier B.V. All rights reserved.
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