期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 93, 期 1, 页码 16-22出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2008.07.001
关键词
Phytochrome; Biliprotein; Photoreceptor; Interaction
资金
- Deutsche Forschungsgemeinschaft [Sfb 498 (B), La 799/7-3]
Phytochromes are widely distributed photoreceptors that are converted by light between the red absorbing Pr and the far-red absorbing Pfr form. The soil bacterium Agrobacterium tumefaciens contains two phytochromes, Agp1 and Agp2, which act as light-regulated histidine kinases. Whereas most phytochromes are stable in the Pr form, Agp2 and few other phytochromes convert into Pfr in darkness. We have shown in a previous publication that the spectral properties of recombinant Agp2 are modified by compounds of the cell extract from an Agrobacterium agp1(-)/agp2(-) double knockout mutant. In the present work we performed concentration series which show that the interaction is specific and that the modifying factor has a concentration of ca. 0.2 mu M. We have also performed a series of mixing experiments with the truncated protein Agp2-M2, which consists of the N-terminal chromophore module (501 amino acids). The cell extract inhibited the photoconversion of Agp2-M2 in an unspecific way. In concentration series, this negative effect was less pronounced when lower concentrations of Agp2-M2 were used. In the presence of excess Agp2-M2 apoprotein, the cell extract did no longer modify the spectral properties of Agp2. The data suggest that the factor of the cell extract interacts specifically with the N-terminal moiety of Agp2. (C) 2008 Elsevier B.V. All rights reserved.
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