Platinum drugs binding to human serum albumin: Effect of non-steroidal anti-inflammatory drugs

In this report, we provide investigations about the effect of Sudlow's site I and II blockers on the platinum anticancer drugs binding. The effect of warfarin, aspirin, ibuprofen and meloxicam on the platinum binding to HSA has been investigated through gel-filtration chromatography, UV-vis, CD, fluorescence spectroscopy and the inductively coupled plasma atomic emission spectroscopy (ICP(AES)) method. The results confirm that the platinum complexes bind to Sudlow's site I and II. Modifications of the protein structure caused by non-steroidal anti-inflammatory drugs (NSAIDs) that bind in these areas have an impact on the amount of cisplatin bound to albumin. The number of bound cisplatin was reduced by approximately 30-40% compared to the native protein. (C) 2014 Elsevier B.V. All rights reserved.