期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
卷 264, 期 -, 页码 41-47出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2013.05.003
关键词
BODIPY-imidazolium salt; Fluorescent intercalator; DNA; Fluorescent turn-on probe
资金
- National Science Foundation of China [20902021, 21172071, 21190033]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars (State Education Ministry)
- Fundamental Research Funds for the Central Universities
The design of novel small molecules for studying the interaction with DNA is one of the most important goals in modern medicinal chemistry. In this paper, we developed two BODIPY-imidazolium salts, 1 and 2, as sensitive and selective fluorescent intercalators toward DNA. The nature and the strength of the stacking interaction between these BODIPYs and DNA has been addressed through a detailed study of the photophysical properties of the bound form and unbound form of the dyes. Strong hypochromism and red-shifted absorption spectra, together with the marked decrease in the positive CD band of ct-DNA, are consistent with strong intercalation of the chromophores with DNA. More important, these dyes give substantial increases in fluorescence on DNA binding and may prove useful as sensitive fluorescent turn-on probes for DNA. The time-resolved fluorescence shows single exponential decay with longer lived excited-state lifetime observed upon intercalative interaction. The intercalation binding model is also supported by steady-state emission quenching experiments using KI as a quencher. (c) 2013 Elsevier B.V. All rights reserved.
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