期刊
JOURNAL OF PHARMACY AND PHARMACOLOGY
卷 67, 期 5, 页码 666-672出版社
WILEY-BLACKWELL
DOI: 10.1111/jphp.12369
关键词
DPPH; myeloperoxidase; polysorbates; ROS; Tween
资金
- Lidervet S.L. (Tarragona, Spain)
- Generalitat de Catalunya
- European Social Fund
ObjectivesThis study aimed to evaluate the possible antioxidant activity of Tween-20 and Tween-80, two amphipathic nonionic surfactants commonly used as solubilizers and stabilizers, whose pharmacological effects have been ignored to a large degree. MethodsAntioxidant activity was investigated in vitro measuring the scavenging activity on the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH.), the production of reactive oxygen species (ROS) in activated human neutrophils using flow cytometry and the myeloperoxidase (MPO) inhibitory activity. Key findingsTween-20 and Tween-80 did not show scavenging activity on DPPH., while produced a decrease of the ROS production in human neutrophils, being Tween-20 more active than Tween-80. Moreover, Tween-80 and Tween-20 were found to significantly stimulate MPO enzymatic activity. ConclusionsOur findings raise concerns with regard to the indiscriminate use of Tween-20 and Tween-80 in clinical and laboratory testing, since they could influence the results that are assigned to the tested substance.
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