Extracts from Epilobium sp herbs induce apoptosis in human hormone-dependent prostate cancer cells by activating the mitochondrial pathway

Objectives The aim of this work was to determine the effect of standardized aqueous extracts from Epilobium angustifoliumL., E.parviflorumSchreb. and E.hirsutumL. herbs on the apoptosis of hormone-dependent prostate cancer cells (LNCaP). Methods The extracts were characterized using high-performance liquid chromatography-diode array detector coupled with mass spectrometry (HPLC-DAD-MS/MS). Apoptosis in the cells was analysed using Annexin V-fluorescein isothiocyanate, and mitochondrial potential, m, using JC-1 by flow cytometry. Caspase-3 activity was determined by enzyme-linked immunosorbent assay. Key findings Using the HPLC-DAD-MS/MS method, 38 constituents were characterized. Extracts contained significant amounts of oenothein B as well as flavonoids and phenolic acids. Exposure of LNCaP cells to the extracts (20, 50 and 70g/ml) resulted in a significant increase in the level apoptotic cells, from 2.86 +/- 0.5% (for untreated cells) up to 86.6 +/- 1.0%. All extracts significantly decreased the mitochondrial potential, m, resulting in an increase in the activity of caspase-3 from 0.3 +/- 0.07ng/mg of protein (for untreated cells) up to 1.26 +/- 0.32ng/mg of protein. Conclusions This study demonstrated that Epilobium extracts are active against LNCaP prostate cancer cells and that their apoptotic activity is related to activation of the mitochondrial pathway. The high oenothein B content may influence the biological activity of these plant materials.