期刊
JOURNAL OF PHARMACY AND PHARMACOLOGY
卷 60, 期 9, 页码 1227-1236出版社
PHARMACEUTICAL PRESS-ROYAL PHARMACEUTICAL SOC GREAT BRITIAN
DOI: 10.1211/jpp.60.9.0016
关键词
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资金
- Pukyong National University
- Food & Drug Administration, South Korea
Important targets for the prevention and treatment of diabetic complications include aldose reductase (AR) inhibitors (ARIs) and inhibitors of advanced glycation endproduct (AGE) formation. Here we evaluate the inhibitory activities of prenylated flavonoids isolated from Sophora flavescens, a traditional herbal medicine, on rat lens AR (RLAR), human recombinant AIR (HRAR) and AGE formation. Among the tested compounds, two prenylated chalcones - desmethylanhydroicaritin (1) and 8-lavandulylkaempferol (2) - along with five prenylated flavanones - kurarinol (8), kurarinone (9), (2S)-2'-methoxykurarinone (10), (2S)-3 beta, 7,4'-trihydroxy-5-methoxy-8-(gamma, gamma-dimethylally)-flavanone (11), and kushenol E (13) were potent inhibitors of RLAR, with IC50 values of 0.95, 3.80, 2.13, 2.99, 3.77, 3.63 and 7.74 mu M, respectively, compared with quercetin (IC50 7.73 mu M). In the HRAR assay, most of the prenylated flavonoids tested showed marked inhibitory activity compared with quercetin (IC50 2.54 mu M). In particular, all tested prenylated flavonols, such as desmethylanhydroicaritin (1, IC50 0.45 mu M), 8-lavandulylkaempferol (2, IC50 0.79 mu M) and kushenol C (3, IC50 0.85 mu M), as well as a prenylated chalcone, kuraridin (5, IC50 0.271 mu M), and a prenylated flavanone, (2S)-7,4'-dihydroxy-5-methoxy-8-(gamma, gamma-dimethylally)-flavanone (12, IC50 0.37 mu M), showed significant inhibitory activities compared with the potent AR inhibitor epalrestat (IC50 0.28 mu M). Interestingly, prenylated flavonoids 1 (IC50 104.3 mu g mL(-1)), 2 (IC50 132.1 mu g mL(-1)), 3 (IC50 84.6 mu g mL(-1)) and 11 (IC50 261.0 mu g mL(-1)), which harbour a 3-hydroxyl group, also possessed good inhibitory activity toward AGE formation compared with the positive control aminoguanidine (IC50 115.7 mu g mL(-1)). Thus, S. flavescens and its prenylated flavonoids inhibit the processes that underlie diabetic complications and related diseases and may therefore have therapeutic benefit.
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