期刊
JOURNAL OF PHARMACOLOGICAL SCIENCES
卷 125, 期 4, 页码 394-405出版社
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.14060FP
关键词
IL-17A; macrophage; iNOS; intestinal inflammation; smooth muscle
资金
- Japanese Ministry of Education [24248050, 25660224, 25252055]
- Grants-in-Aid for Scientific Research [25660224, 25252055, 24500501] Funding Source: KAKEN
Intestinal inflammation causes disorder in bowel motility. Th17 cytokines are involved in intestinal inflammation. To understand the role of interleukin (IL)-17 in intestinal motility, we examined effects of IL-17A on contractile activities of organ-cultured ileum. Rat ileal smooth muscle strips were organ cultured with IL-17A. Muscle contraction was measured, and cells expressing inducible nitric oxide synthase (iNOS) were identified with immunohistochemistry. Creating Th17-transferred colitis model mice, in vivo effects of IL-17 on contractile activities, and iNOS mRNA expression in colonic smooth muscle were investigated. Treatment with IL-17A for 12 h and 3 days attenuated carbachol-and membrane depolarization induced contractions in organ-cultured rat ileum. N-G-Nitro-L-arginine methyl ester (100 mu M), a nitric oxide synthase inhibitor, completely reversed the IL-17A induced inhibition of contractile force. Ileal tissue cultured in the presence of IL-17A showed increased expression of iNOS mRNA and protein. Immunohistochemical analysis using an iNOS antibody revealed that iNOS protein was expressed on ED2-positive muscularis macrophages. The level of iNOS mRNA was also increased in inflamed colonic smooth muscle of Th17-transferred colitis model mice. In intestinal inflammation, IL-17A induces an intestinal motility disorder through iNOS expression in muscularis macrophages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据