期刊
JOURNAL OF PHARMACEUTICAL SCIENCES
卷 98, 期 1, 页码 268-280出版社
ELSEVIER SCIENCE INC
DOI: 10.1002/jps.21412
关键词
polyelectrolyte complex; fibroblast growth factor; nanoparticle; controlled release; stability
资金
- American Heart Association
- Juvenile Diabetes Research Foundation
- NIH [P20 RR016443, R03 AR054035]
- Department of Defense [(W81XWH-07-1-0021]
- Cystic Fibrosis Foundation
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR016443] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R03AR054035] Funding Source: NIH RePORTER
The therapeutic value of many growth factors is often hindered by the narrow therapeutic index and sustained concentrations required for efficacy. Controlled release approaches provide a valuable tool to achieve these goals; however, growth factor stability must be maintained. Repifermin (R) is a truncated form of fibroblast growth factor-10, also known as keratinocyte growth factor-2, that exhibits promise in wound healing applications; however, controlled release formulation presents a challenge for this labile protein. Taking advantage of the heparin-binding motif of this class of biopharmaceuticals, Repifermin (R) was effectively stabilized and packaged in polyelectrolyte complexes. In the presence of dextran sulfate, the unfolding temperature of this growth factor was increased by similar to 10 degrees C as confirmed by a variety of spectroscopic techniques. Dextran sulfate with bound Repifermin (R) was then complexed with several polycations (chitosan, poly-L-lysine, and polyethylenimine) resulting in the formation of similar to 250 nm polyelectrolyte complexes that entrapped the protein with similar to 70-80% efficiency. Release was controlled for more than 10 days and the mitogenic activity of Repifermin (R) on human umbilical cord vascular endothelial cells was significantly enhanced, whereas no effect was noted for free Repifermin (R). (C) 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 98:268-280, 2009
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据