期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 161, 期 -, 页码 262-272出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2018.08.039
关键词
Naringin; Metabolite; Human intestinal microbe; Stable isotope-labeling; UFLC-Q-TOF-MS/MS
资金
- fund of National Major Scientific and Technical Special Project of China [2015ZX09101014]
- Applied Science and Technology R & D Special Fund Project of Guangdong Province [2015B020234004]
- Fundamental Research Funds for the Central Universities [20173300031610135]
- Science, Technology and Innovation Commission of Shenzhen Municipality [JCYJ20160530153822178]
Widely presented in medicinal plants, naringin is one of the major flavanones with various pharmaceutical bioactivities. After oral administration, naringin predominantly undergoes metabolisms mediated by liver cytochrome P450 and gut microbes, while its human microbes-mediated metabolic profiling is still largely obscure due to the endogenous interferences, which makes it extremely difficult to analyze metabolites precisely. In this study, we aim of systematically investigating the biotransformation of naringin mediated by human intestinal microbes through applying stable isotope-labeling method. [2',3',5',6'-D-4]naringin was synthesized and incubated anaerobically with human gut microbes. A total of 13 microbial metabolites were detected and identified by UFLC-Q-TOF-MS/MS, among which 5 were reported for the first time. Furthermore, the proposed metabolic pathway revealed that naringin went through extensive phase I metabolism in human intestinal microbes. Of note, diverse metabolic profiles of naringin among human participants were obtained, which could be attributed to the distinct gut microbiota compositions of individuals. (C) 2018 Elsevier B.V. All rights reserved.
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