期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 87, 期 -, 页码 155-166出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2013.07.021
关键词
Bioaffinity-based strategies; Protein ligand screening; Zonal chromatography; Frontal chromatography; Nonlinear chromatography
资金
- National Council for Scientific and Technological Development (CNPq)
- Brazilian Agency for support and Evaluation of Graduate Education (CAPES)
- Sao Paulo State Research Foundation (FAPESP) [2008/04371-5]
- National Institute of Science and Technology (INCT) - Controle Biorracional de Insetos Praga (CBIP)
The diversity of small molecules available to produce truly innovative drugs associated with the wealth of known biological targets calls for key strategies in protein ligand screening. This review encompasses the recently developed bioaffinity-based strategies. A critical view of the use of zonal, frontal, and nonlinear chromatography with immobilized proteins is given. The association of these elution modes with the ligand fishing method, which uses nanomagnetics particles, is also addressed. A series of applications and how these new screening strategies can be used to determine the function, affinity, and activity parameters of proteins is discussed. (C) 2013 Elsevier B.V. All rights reserved.
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