期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 86, 期 -, 页码 189-197出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2013.08.010
关键词
Flos Lonicerae; Phenolic acids; UPLC-MS/MS; Oral delivery; Pharmacokinetics
资金
- National Natural Science Foundation of China [81073071, 81273655]
- Qing Lan Project from Jiangsu Provincial Technology Innovation Team Support Scheme
- priority Academic Program Development of Jiangsu Higher Education Institution [ysxk-2010]
- program sponsored for scientific innovation research of college graduate in Jiangsu province [623]
The current study aims to investigate the pharmacokinetic study of five phenolic acids (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid) following oral administration of Flos Lonicerae preparations in rats. A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to simultaneously determine the five phenolic acids in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid-liquid extraction with ethyl acetate/n-hexane (9:1, v/v). The separation was performed on an Acquity UPLC BEH C18 column (100 mm x 2.1 mm, 1.7 mu m) at a flow rate of 0.4 ml min(-1), and acetonitrile/methanol (4:1, v/v)-0.4% formic acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive ionization mode. All calibration curves had good linearity (r > 0.991) over the concentration ranges of 0.74-378 ng ml(-1) for neochlorogenic acid, 0.50-1030 ng ml(-1) for chlorogenic acid, 1.9-250 ng ml(-1) for cryptochlorogenic acid, 0.74-380 ng ml(-1) for 3,5-dicaffeoylquinic acid, and 5.1-328 ng ml(-1) for 3,4-dicaffeoylquinic acid. The intra-and inter-day precision were within 15% and the accuracy ranged from 86.2% to 114.1%. (C) 2013 Elsevier B.V. All rights reserved.
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