期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 58, 期 -, 页码 49-57出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2011.09.032
关键词
Aminoglycosides; CE-C(4)D; Tobramycin; Pharmaceutical analysis; Quality control
资金
- Faculty of Pharmacy, University of Tripoli, Tripoli, Libya
A method was validated and optimized to determine tobramycin (TOB) and its related substances. TOB is an aminoglycoside antibiotic which lacks a strong UV absorbing chromophore or fluorophore. Due to the physicochemical properties of TOB, capillary electrophoresis (CE) in combination with Capacitively Coupled Contactless Conductivity Detection (C(4)D) was chosen. The optimized separation method uses a background electrolyte (BGE) composed of 25 mM morpholinoethane-sulphonic acid (MES) adjusted to pH 6.4 by L-histidine CL-His). 0.3 mM cetyltrimethyl ammonium bromide (CTAB) was added as electroosmotic flow modifier in a concentration below the critical micellar concentration (CMC). Ammonium acetate 50 mg L(-1) was used as internal standard (IS). 30 kV was applied in reverse polarity (cathode at the injection capillary end) on a fused silica capillary (65/43 cm; 75 mu m id). The optimized separation was obtained in less than 7 min with good linearity (R(2) = 0.9995) for tobramycin. It shows a good precision expressed as RSD on relative peak areas equal to 0.2% and 0.7% for intraday and interday respectively. The LOD and LOQ are 0.4 and 1.3 mg L(-1) corresponding to 9 pg and 31 pg respectively. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据