期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 70, 期 -, 页码 492-498出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2012.07.004
关键词
Antidepressant drugs; High performance liquid chromatography; SnO2 nanorods; Solid phase microextraction; Trace analysis
资金
- International Centre for Science, High Technology & Environmental Sciences [1/3937]
The arrays of tin oxide nanorods-solid phase microextraction (ATN-SPME) fibre coupled with the high performance liquid chromatography (HPLC) method was developed for simultaneous determination of selective serotonin reuptake inhibitors (SSRI), Citalopram and Fluoxetine, in human urine and plasma samples. The variables of interest in the Direct-SPME (D-SPME) were extraction time, pH, ion strength or salt percentage and desorption time of analytes from the fibre. These factors were optimised by using a Box-Behnken design and the response surface equations were developed. The optimal experimental conditions obtained from this statistical evaluation included: the salt percentage (30%, w/v), NaOH volume (6.5 mu l from a 1M solution), extraction time (10 min) and desorption time (30 min) for drugs in the plasma sample and The salt percentage (30%, w/v), NaOH volume (100 mu l from a 1 M solution), extraction time (18 min) and desorption time (23 min) for drugs in the urine sample. A satisfactory reproducibility for the extraction from urine and plasma samples (R.S.D. < 10%) was obtained. The linearity for urine and plasma ranged from 1 to 5 x 10(5) ng ml(-1) with a detection limit of 0.2 ng ml(-1) for Citalopram and 0.5 ng ml(-1) for Fluoxetine, which covered the typical urinary concentrations obtained for Citalopram and Fluoxetine. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据