期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 53, 期 1, 页码 43-49出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2010.03.021
关键词
Ultra performance liquid chromatography (UPLC); Method validation; Curcuma longa Linn.; Quality control; Curcuminoids
资金
- 973 Program of China [2006CB504703]
- National Natural Science Fund of China [30600824]
A new reversed phase ultra performance liquid chromatography (UPLC) method was developed for the rapid quantification of three curcuminoids (curcumin (C), desmethoxycurcumin (DMC) and bisdesmethoxycurcumin (BDMC)) in Curcuma longa Linn. (C. longa) using a Waters BEH Shield RP C-18, 2.1 mm x 100 mm, 1.7 mu m column. The runtime was 2 min. The influence of column temperature and mobile phase on resolution was investigated. The method was validated according to the ICH guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The limits of detection were 40.66, 49.38 and 29.28 pg for C, DMC and BDMC, respectively. Limits of quantitation for C, DMC and BDMC, were 134.18, 164.44 and 97.50 pg. respectively. Linear range was from 3.28 to 46.08 mu g/ml. The mean +/- SD percent recoveries of curcuminoids were 99.47 +/- 1.66, 99.50 +/- 1.99 and 97.77 +/- 2.37 of C, DMC and BDMC, respectively. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The proposed method was found to be reproducible and convenient for quantitative analysis of three curcuminoids in C. longa. This work provided some references for quality control of C. longa. (C) 2010 Elsevier B.V. All rights reserved.
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