Characterization of homoisoflavonoids in different cultivation regions of Ophiopogon japonicus and related antioxidant activity

Homoisoflavonoids were identified in Ophiopogon japonicas of two cultivation regions in China Hangmaidong (grown in Zhejiang province) and Chuan-maidong (grown in Sichuan province). Liquid chromatography (LC). coupled with electrospray ionization (ESI) tandem mass spectrometry (MS), was developed to analyze homoisoflavonoids in these two areas. Based on LC-MS/MS data, 24 homoisoflavonoid compounds-19 in Hang-maidong and 17 in Chuan-maidong were identified or tentatively characterized The homoisoflavonoids in the two regions were similar in chemical profile, but distinctive in their combination and ratio Methylophiopogonanone A and methylophiopogonanone B were the major contributors to the total homoisoflavonoid content Hang-maidong had a higher total homoisoflavonoid content and better DPPH (2,2-diphenyl-1-pieryl-hydrazyl) radical-scavenging activity than that of Chuan-maidong. The total phenolic content showed no significant difference between the two regions. This study allows a clear chemical differentiation of Hang-maidong from Chuan-maidong (C) 2010 Elsevier B V All rights reserved