期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 51, 期 1, 页码 39-45出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2009.07.031
关键词
FTIR; Secondary structure; Protein formulation; Circular dichroism; Multivariate data analysis; Calibration curve
The aim of this study was to develop a quick and objective method for the determination of changes in protein secondary structure by Fourier transform infrared spectroscopy (FTIR). Structural shifts from native regions (alpha-helix, intramolecular beta-sheet) to aggregated strands (intermolecular beta-sheet) were used to evaluate protein damage. FTIR spectra of 16 different proteins were recorded and quantified by peak fitting of the non-deconvolved and baseline corrected amide I bands. The resulting percentile secondary structures were correlated with the shape and intensity of the area normalized amide I bands using an interval partial least squares algorithm (iPLS). Structural elements were focused on the following regions: alpha-helix 1660-1650 cm(-1), intramolecular beta-sheet 1695-1683 cm(-1) and 1644-1620 cm(-1), intermolecular beta-sheet 1620-1595 cm(-1). Three calibration curves were created from the data sets. Calculated alpha-helix content ranged from 0% to 79.59%, intramolecular beta-sheet from 10.64% to 63.89% and intermolecular beta-sheet from 0.23% to 9.70%. The linear relationship between actual values (as determined by peak fitting) and calculated values was evaluated by correlation coefficient and root mean square error of calibration while cross-validation was performed to detect possible outliers. Results were verified by including two proteins as validation standards and comparing the calculated values to peak fitting and X-ray data. Structural changes of human serum albumin (HSA) due to elevated temperatures and the fibrillation of glucagon were quantified by calibration curve analysis. Performance and reliability of the iPLS algorithm were evaluated by comparing calculated secondary structure elements with results from peak fitting and circular dichroism. Different methods for the determination of secondary structure gave slightly different results but overall tendencies concurred. Additionally, formation of HSA aggregates could be linked to increasing beta-sheet content by comparing SEC-HPLC and turbidity analysis with results from the FTIR calibration curves. In summary, quantification of the alpha-helix to beta-sheet transition by iPLS analysis proves to be a feasible and objective way for the determination of damage to protein secondary structure. (C) 2009 Elsevier B.V. All rights reserved.
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