期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 52, 期 4, 页码 615-619出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2010.01.030
关键词
On-line; Molecular imprinted; Solid-phase extraction; Florfenicol; Flow-injection fluorescence
资金
- National Natural Science Foundation of China [50972050]
- Science Research Foundation of Shandong Province, China [Y2007B07]
- Science Research Foundation of University of Jinan, China [XKY0817]
A simple and convenient on-line molecular imprinted solid-phase extraction flow-injection fluorescence sensor was developed in this study. Florfenicol (FF)-imprinted polymer was prepared by self-assembly with acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinker. The binding characteristics of the imprinted polymer to florfenicol were evaluated by equilibrium binding experiments, and the morphology of the imprinted polymer was studied by scanning electron microscope (SEM). Fluorescence intensity of 3-p-nitrylpheny1-5-(2'-sulfonophenylazo) rhodanine (4NRASP) and bovine serum albumin (BSA) was inhibited by FF. Under optimum conditions the intensity of fluorescence shows a linear relationship with the concentration of FF over the range of 1.2 x 10(-6) to 2.6 x 10(-5) g mL(-1) with a regression equation of Delta I= 23.54 + 17.86c (c 10(-6) g mL(-1)) (r = 0.9965, n = 6). The low detection limit of FF was found to be 3.4 x 10(-7) g mL(-1) according to 11 parallel determinations for the blank solution. The relative standard deviation for the determination of 2.0 x 10(-6) g mL(-1) FE solution was 3.5% (n = 11). This proposed sensor could be satisfactorily applied to the determination of FF in liver and meat samples. (C) 2010 Elsevier BVAll rights reserved.
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