期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 51, 期 1, 页码 178-185出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2009.07.015
关键词
SBSE; LC; Fluoxetine; Citalopram; Venlafaxine; Metabolites; Plasma; Urine and brain tissue samples
资金
- Spanish Minister of Science and Innovation [CTQ2008-00651/BQU]
The aim of this article is to present an analytical application of stir bar sorptive extraction (SBSE) coupled to HPLC-fluorescence detection (FLD) for the quantification of fluoxetine (FIX), citalopram (CIT) and venlafaxine (VLF) and their active metabolites - norfluoxetine (NFLX), desmethyl- (DCIT) and didesmethylcitalopram (DDCIT) and o-desmethylvenlafaxine (ODV) - in plasma, urine and brain tissue samples. All the parameters influencing adsorption (pH, ion strength, organic modifier addition, volume, extraction time and temperature) and desorption (desorption solvent composition, time, temperature and desorption mode) of the analytes on the stir bar have been optimized. For each matrix, the analytical method has been assessed by studying the linearity and the intra- and interday accuracy (89-113%) and precision (RSD < 13%). The improvement of the quantification limits (0.2-2 mu g l(-1) for plasma, 2-20 ng g(-1) for brain tissue and 1-10 mu g l(-1) for urine, depending on the respective response for analytes) and the development of a procedure for all the matrices make this method useful in clinical and forensic analysis. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据