期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 49, 期 4, 页码 1123-1127出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2009.02.014
关键词
Anthraquinones; HPLC-fluorescence detection; HPLC-UV detection; Traditional Chinese medicine; Pharmaceutical preparation
资金
- 973 project [2006CB504701]
- National Natural Science Foundation ofChina [20875028]
- Science Research Foundation of Hunan [2007FJ1005]
A reversed-phase high-performance liquid chromatography (RP-HPLC) method with fluorescence detection for simultaneous determination of five anthraquinones in Rhubarb collected from nine different locations in China, Polygonum cuspidatum, Polygoni multiflori and three pharmaceutical preparations is proposed and validated. Chromatography was carried out at 25 degrees C on a Hypersil C18 column with the isocratic mobile phase of methanol-0.1% aqueous formic acid (85:15, v/v) at a flow rate of 1.0 ml/min. The excitation and emission wavelengths were set at 440 and 540 nm, respectively. A comprehensive validation of the method included tests of sensitivity, linearity, precision and accuracy. The linear regressions were acquired with r > 0.999. Satisfactory intra- and inter-day precisions were achieved with R.S.D.s less than 3.95% and the average recovery factors obtained were in the range of 93.2-103.8%. (C) 2009 Elsevier B.V. All rights reserved.
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