Determination of suxamethonium in a pharmaceutical formulation by capillary electrophoresis with contactless conductivity detection (CE-C4D)

A simple method based on capillary electrophoresis with a capacitively coupled contactless conductivity detector (CE-(CD)-D-4)) was developed for the determination of suxamethonium (SUX) in a pharmaceutical formulation. A hydro-organic mixture, consisting of 100 mM Tris-acetate buffer at pH 4.2 and acetonitrile (90: 10, v/v), was selected as background electrolyte (BGE). The applied voltage was 30 kV, and the sample injection was performed in the hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50 mu m and a total length of 64.5 cm. Under these conditions, a complete separation between SUX, sodium ions and the main degradation products (choline) was achieved in less than 4 min. The presence of acetonitrile in the BGE allowed a reduction of SUX adsorption on the capillary wall. The CE-(CD)-D-4) method was validated, and trueness values between 98.8% and 101.1% were obtained with repeatability and intermediate precision values of 0.7-1.3% and 1.2-1.6%, respectively. Therefore, this method was found appropriate for controlling pharmaceutical formulations containing suxamethonium and degradation products. (c) 2008 Elsevier B.V. All rights reserved.