期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 46, 期 3, 页码 577-582出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jpba.2007.11.008
关键词
carboxylic acids; capillary electrophoresis; Garcinia atroviridis Griff; hydroxycitric acid; hydroxycarboxylic acid
Capillary zone electrophoresis (CZE) was developed for quantitative determination of hydroxycitric acid and hydroxycitric acid lactone in herbal products of Garcinia atroviridis Griff. Resolution optimization was investigated by varying type, concentration and pH of buffers. Using the pH 9.2 buffer containing 30 mM Na2B4O7, 90 mM NaH2PO4 and 0.5 mM tetradecyltrimethyl ammonium bromide, baseline resolution (R-s > 1.5) was found for all analytes. Advantages of the developed CZE method include simple sample preparation, fast analysis time within 5 min and high accuracy and precision. (c) 2007 Elsevier B.V. All rights reserved.
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