期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 48, 期 3, 页码 909-917出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jpba.2008.07.013
关键词
Gastrodia elata Blume; Gastrodin; Herbal medicine; Microdialysis; Pharmacokinetics
资金
- National Science Council, Taiwan [NSC96-2113-M-010-003-MY3, NSC96-2628-B-010-006-MY3]
Gastrodin is a pharmacologically active substance isolated from Gastrodia elata Blume with sedation, anti-convulsion and anti-epilepsy activities. A rapid and sensitive liquid chromatography technique coupled to tandem mass spectrometry (LC-MS/MS) system was developed to determine gastrodin and its metabolite p-hydroxybenzyl alcohol (HBA) in rat blood, brain and bile collected using microdialysis technique. The analytes were separated using a reversed phase column (4.6 mm x 150 mm, 5 mu m). The mobile phase for column separation was 30% methanol with a flow rate of 0.6 mL/min. As a post-column addition, 1% ammonium hydroxide solution (in methanol) was additionally pumped via a T-connection using a chromatographic pump (BAS PM-80, USA) at a flow rate of 0.2 mL/min after the column separation. A LC-MS/MS system equipped with a negative electrospray ionization (ESI) source in multiple reaction monitoring (MIRM) mode was used to monitor m/z 285.0 -> 122.9 and m/z 123.0 -> 105.0 transitions for gastrodin and HBA, respectively. The lower limit of quantification (LLoQ) for gastrodin and HBA were 0.5 and 2 ng/mL, respectively. The calibration curves were linear over the range of 0.5-5000 ng/mL and 2-1000 ng/mL for gastrodin and HBA with a coefficient of determination > 0.995, respectively. This selective and sensitive method is useful for the determination of gastrodin and HBA and in the pharmacokinetic studies of these compounds. (c) 2008 Elsevier B.V. All rights reserved.
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