4.5 Article

Melatonin as a Candidate Therapeutic Drug for Protecting Bone Cells From Chlorhexidine-Induced Damage

期刊

JOURNAL OF PERIODONTOLOGY
卷 85, 期 12, 页码 E379-E389

出版社

WILEY
DOI: 10.1902/jop.2014.140279

关键词

Cell death; cell respiration; chlorhexidine; melatonin; osteoblasts; reactive oxygen species

资金

  1. German Society of Dental, Oral, and Maxillofacial Medicine [DGZMK 06-2014]

向作者/读者索取更多资源

Background: Melatonin was proposed for use in periodontitis and peri-implantitis therapy due to its bonesupportive effects. This issue is of interest because standard adjuvant antiseptics, namely chlorhexidine (CHX), prove damaging for osteoblasts. Thus, the aim of this study is to investigate if melatonin is suitable as an auxiliary agent for protecting osteoblasts from CHX damage. Methods: MC3T3 osteoblast response was determined following administration of various CHX concentrations in the absence or presence of melatonin. Osteoblast morphology was evaluated, total reactive oxygen species (ROS) and superoxide levels were quantified, ratios of apoptotic and necrotic cells were identified by flow cytometry, metabolic activity of remaining cells was assessed, and effects were calculated with repeated measures analysis and post hoc P value adjustment. Results: CHX led to poor morphology, increased total ROS and superoxide levels, and rigorously diminished the number of vital and metabolic active osteoblasts in a concentration-dependent manner. However, simultaneous melatonin supply supported cell morphogenesis and growth, reduced ROS and superoxide generation, shifted the percentage of CHX-damaged cells from necrotic/late to early apoptotic events, and modulated metabolic activity in osteoblasts. Conclusion: These data reveal that melatonin protects osteoblasts in the CHX context, thereby implicating melatonin as a promising drug in periodontitis and peri-implantitis treatment.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据