期刊
JOURNAL OF PERIODONTOLOGY
卷 85, 期 8, 页码 1070-1080出版社
AMER ACAD PERIODONTOLOGY
DOI: 10.1902/jop.2013.130365
关键词
Aggressive periodontitis; genes; gingival crevicular fluid; matrix metalloproteinase 8; polymorphism; tissue inhibitor of metalloproteinase-1
资金
- Academy of Finland Helsinki, Finland
- Research Foundation of Helsinki University Central Hospital
- Ege University Research Foundation
Background: The aim of the present study is to investigate matrix metalloproteinase (MMP)-8 and tissue inhibitor of MMP-1 (TIMP-1) gene polymorphisms in generalized aggressive periodontitis (GAgP) and to assess the effects of MMP-8 and TIMP-1 genotypes on the outcomes of non-surgical periodontal therapy. Methods: Genomic DNA was obtained from peripheral blood of 100 patients with GAgP and 167 periodontally healthy controls. MMP-8 +17 C/G, -799 C/T, -381 A/G and TIMP-1 372 T/C, *429 T/G polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism. Patients with GAgP received non-surgical periodontal therapy and were followed for 6 months. Clinical periodontal parameters and gingival crevicular fluid (GCF) samples were collected at baseline and at follow-up visits. GCF biomarkers were analyzed by immunofluorescence assay and enzyme-linked immunosorbent assay. Results: Distribution of the MMP-8 -799 C/T genotypes was significantly different between the GAgP and control groups (P < 0.005). TIMP-1 372 T/C and *429 T/G genotypes in males were also significantly different between study groups (P < 0.004). GCF MMP-8 levels decreased until 3 months after non-surgical therapy compared with baseline in T and G alleles, as well as G and C allele carriers (P < 0.0125), whereas no significant decreased was observed in non-carriers (P > 0.0125). Conclusion: On the basis of the present findings, it can be suggested that MMP-8 -799 C/T and TIMP-1 372 T/C, *429 T/G gene polymorphisms in males may be associated with the susceptibility to GAgP in the Turkish population.
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