期刊
JOURNAL OF PERIODONTOLOGY
卷 79, 期 8, 页码 1480-1490出版社
AMER ACAD PERIODONTOLOGY
DOI: 10.1902/jop.2008.070624
关键词
alveolar bone loss; animal study; cell adhesion molecule; periodontitis; periodontium
资金
- NIDCR NIH HHS [R01 DE013397, R01 DE013397-07, R29 DE013480, T32 DE 07294, T32 DE007294-06, DE 13480, DE13397, T32 DE007294, R29 DE013480-05] Funding Source: Medline
Background: The ability of the periodontal ligament (PDL) to absorb and distribute forces is necessary for periodontal homeostasis. This adaptive response may be determined, in part, by a key molecule, periostin, which maintains the integrity of the PDL during occlusal function and inflammation. Periostin is primarily expressed in the PDL and is highly homologous to beta ig-H3 (transforming growth factor-beta [TGF-beta] inducible gene). Cementum, alveolar bone, and the PDL of periostin-null mice dramatically deteriorate following tooth eruption. The purpose of this study was to determine the role of periostin in maintaining the functional integrity of the periodontium. Methods: The periodontia from periostin-null mice were characterized followed by unloading the incisors. The effect of substrate stretching on periostin expression was evaluated using a murine PDL cell line. Real-time reverse transcription-polymerase chain reaction was used to quantify mRNA levels of periostin and TGF-beta. TGF-beta 1 neutralizing antibodies were used to determine whether the effects of substrate stretching on periostin expression are mediated through TGF-beta. Results: Severe periodontal defects were observed in the periostin-null mice after tooth eruption. The removal of masticatory forces in periostin-null mice rescue the periodontal defects. Periostin expression was increased in strained PDL cells by 9.2-fold at 48 hours and was preceded by a transient increase in TGF-beta mRNA in vitro. Elevation of periostin in response to mechanical stress was blocked by the addition of 2.5 ng/ml neutralizing antibody to TGF-beta 1, suggesting that mechanical strain activates TGF-beta to have potential autocrine effects and to increase periostin expression. Conclusion: Mechanical loading maintains sufficient periostin expression to ensure the integrity of the periodontium in response to occlusal load.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据