期刊
JOURNAL OF PERIODONTAL RESEARCH
卷 48, 期 4, 页码 405-410出版社
WILEY-BLACKWELL
DOI: 10.1111/jre.12018
关键词
cementum; root; stabilization; Wnt/beta-catenin signaling
资金
- National Research Foundation of Korea (NRF)
- Korea government (MEST) [2009-0085733, 2011-0027790]
- National Research Foundation of Korea [2009-0085733] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Background and Objective Wingless-type MMTV integration site family (Wnt)/-catenin signaling plays an essential role in cellular differentiation and matrix formation during skeletal development. However, little is known about its role in tooth-root formation. In a previous study, we found excessive formation of dentin and cementum in mice with constitutive -catenin stabilization in the dental mesenchyme. In the present study we analyzed the molar roots of these mice to investigate the role of Wnt/-catenin signaling in root formation in more detail. Material and Methods We generated OC-Cre:Catnb+/lox(ex3) mice by intercrossing Catnb+/lox(ex3) and OC-Cre mice, and we analyzed their mandibular molars using radiography, histomorphometry and immunohistochemistry. Results OC-Cre:Catnb+/lox(ex3) mice showed impaired root formation. At the beginning of root formation in mutant molars, dental papilla cells did not show normal differentiation into odontoblasts; rather, they were prematurely differentiated and had a disorganized arrangement. Interestingly, SMAD family member 4 was upregulated in premature odontoblasts. In 4-wk-old mutant mice, molar roots were about half the length of those in their wild-type littermates. In contrast to excessively formed dentin in crown, root dentin was thin and hypomineralized in mutant mice. Biglycan and dentin sialophosphoprotein were downregulated in root dentin of mutant mice, whereas dentin matrix protein 1 and Dickkopf-related protein 1 were upregulated. Additionally, ectonucleotide pyrophosphatase/phosphodiesterase 1 was significantly downregulated in the cementoblasts of mutant molars. Finally, in the cementum of mutant mice, bone sialoprotein was downregulated but Dickkopf-related protein 2 was upregulated. Conclusion These results suggest that temporospatial regulation of Wnt/-catenin signaling plays an important role in cell differentiation and matrix formation during root and cementum formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据