期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 99, 期 17, 页码 7035-7045出版社
SPRINGER
DOI: 10.1007/s00253-015-6651-9
关键词
Catharanthus roseus; Cambial meristematic cells; beta-Cyclodextrin; Methyl jasmonate; Terpenoid indole alkaloids
资金
- National Natural Sciences Foundation of China [81073004, 81102771, 81274045]
- Pearl River Scientific and Technological New Star Program of Guangzhou [2014 J2200004]
- Natural Science Foundation of Guangdong Province [2014A030313385]
- Science and Technology Specific Project of Guangzhou [201300000138]
Long-term stable cell growth and production of vindoline, catharanthine, and ajmalicine of cambial meristematic cells (CMCs) from Catharanthus roseus were observed after 2 years of culture. C. roseus CMCs were treated with beta-cyclodextrin (beta-CD) and methyl jasmonate (MeJA) individually or in combination and were cultured both in conventional Erlenmeyer flasks (100, 250, and 500 mL) and in a 5-L stirred hybrid airlift bioreactor. CMCs of C. roseus cultured in the bioreactor showed higher yields of vindoline, catharanthine, and ajmalicine than those cultured in flasks. CMCs of C. roseus cultured in the bioreactor and treated with 10 mM beta-CD and 150 mu M MeJA gave the highest yields of vindoline (7.45 mg/L), catharanthine (1.76 mg/L), and ajmalicine (58.98 mg/L), concentrations that were 799, 654, and 426 % higher, respectively, than yields of CMCs cultured in 100-mL flasks without elicitors. Quantitative reverse transcription (RT)-PCR showed that beta-CD and MeJA upregulated transcription levels of genes related to the biosynthesis of terpenoid indole alkaloids (TIAs). This is the first study to report that beta-CD induced the generation of NO, which plays an important role in mediating the production of TIAs in C. roseus CMCs. These results suggest that beta-CD and MeJA can enhance the production of TIAs in CMCs of C. roseus, and thus, CMCs of C. roseus have significant potential to be an industrial platform for production of bioactive alkaloids.
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