期刊
JOURNAL OF PEDIATRIC SURGERY
卷 43, 期 6, 页码 1018-1024出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.jpedsurg.2008.02.025
关键词
short gut syndrome; surgery models; mice; adaptation
资金
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R24DK064403, R01DK053234, T32DK007727, R01DK059288] Funding Source: NIH RePORTER
- NIDDK NIH HHS [R01 DK059288-05, R01 DK059288, R01 DK053234, T32 DK007727, R24 DK064403, R01 DK53234, R01 DK59288, R01 DK053234-09, T32 DK07727] Funding Source: Medline
Purpose: Central to the use of murine models of disease is the ability to derive reproducible data. The purpose of this study was to determine factors contributing to variability in our murine model of small bowel resection (SBR). Methods: Male C57B1/6 mice were randomized to sham or 50% SBR. The effect of housing type (pathogen-free vs standard housing), nutrition (reconstituted powder vs tube feeding formulation), and correlates of intestinal morphology with gene expression changes were investigated. Multiple linear regression modeling or I-way analysis of variance was used for data analysis. Results: Pathogen-free mice had significantly shorter ileal villi at baseline and demonstrated greater villus growth after SBR compared to mice housed in standard rooms. Food type did not affect adaptation. Gene expression changes were more consistent and significant in isolated crypt cells that demonstrated adaptive growth when compared with crypts that did not deepen after SBR. Conclusion: Maintenance of mice in pathogen-free conditions and restricting gene expression analysis to individual animals exhibiting morphologic adaptation enhances sensitivity and specificity of data derived from this model. These refinements will minimize experimental variability and lead to improved understanding of the complex process of intestinal adaptation. (c) 2008 Elsevier Inc. All rights reserved.
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