期刊
JOURNAL OF PATHOLOGY
卷 223, 期 5, 页码 683-694出版社
WILEY-BLACKWELL
DOI: 10.1002/path.2834
关键词
PMEPA1; p21; c-Myc; Smad3; prostate cancer
资金
- Department of Defense [W81XWH-07-1-0191]
- American Cancer Society [RSG-08-199-01]
- Yunnan Province High-Profile Talents Project [2010 CI 114]
- American Urological Association Foundation
The PMEPA1 gene has been shown to suppress the androgen receptor (AR) and TGF beta signaling pathways and is abnormally expressed in prostate tumours. However, the role and mechanism action of PMEPA1 in AR-negative prostate cancer are unclear. Here, we demonstrate that inhibition of PMEPA1 suppresses AR-negative RWPE1 and PC-3 prostate cell proliferation through up-regulating the p21 transcription. Additionally, PMEPA1 overexpression suppresses the p21 expression and promotes cell proliferation. PMEPA1 is induced by TGF beta as a negative feedback loop to suppress Smad3 phosphorylation and nuclear translocation; up-regulates c-Myc; down-regulates p21; and promotes PC-3 cell proliferation. The PMEPA1 functions depend on its Smad2/3 binding motif. Consistently, depletion of Smad3/4, but not Smad2, blocks PMEPA1's functions of regulating c-Myc and p21. Importantly, stable depletion of PMEPA1 in PC-3 inhibits xenograft growth. Finally, we found that PMEPA1 is overexpressed in a subset of prostate cancer cell lines and tumours. These findings suggest that PMEPA1 may promote AR-negative prostate cancer cell proliferation through p21. Copyright. (C) 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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