期刊
JOURNAL OF ORTHOPAEDIC RESEARCH
卷 30, 期 9, 页码 1447-1452出版社
WILEY
DOI: 10.1002/jor.22094
关键词
tendinopathy; pain; NMDAR1; phospho-NMDAR1; substance P
类别
资金
- Stockholm County Council
- Karolinska Institutet [SLL20070317]
- Swedish National Centre for Sports Research
- Norwegian South-Eastern Health Corporate
- Royal Norwegian Ministry of Culture
- Norwegian Olympic Committee & Confederation of Sport
- International Olympic Committee
Tendinopathy, pain, and degeneration, may be related to the up-regulation of substance P (SP) and its activation of glutamate receptors. We hypothesized that the pathogenesis of tendinopathy involves N-methyl-D-aspartate receptor type 1 (NMDAR1) activation (phosphorylated NMDAR1; phospho-NMDAR1) co-existing with SP. Moreover, we examined the presence of metabotropic receptors that increase (mGluR1 and mGluR5) or decrease (mGluR6 and mGluR7) NMDAR1 excitability. Biopsies from patients with patellar tendinopathy (n?=?10) and from controls (n?=?8) were immunohistochemically analyzed according to the occurrence and tissue distribution of NMDAR1, phosho-NMDAR1, mGluR (1, 57), and SP. The biopsies were immunohistochemically single- and double-stained and semi-quantitatively assessed. Tendinopathic biopsies exhibited increased occurrence of NMDAR1, phospho-NMDAR1, SP, and mGluR5, while mGluR67 were not increased and mGluR1 was not found. The occurrence of NMDAR1 and SP correlated in tendinopathy (r2?=?0.54, p?=?0.03), but not in the controls (r2?=?0.11, p?=?0.4). Co-localization of SP and phospho-NMDAR1 within the tendon proper was only found in tendinopathy, localized on hypertrophic tenocytes, blood vessels, and penetrating free-nerve fibres. Up-regulation and activation of the glutamate receptor, phospho-NMDAR1, suggests a role in the pathophysiology of tendinopathy. Increased NMDAR1 excitability may be related to increased SP and mGluR5. The unique co-existence of SP and phospho-NMDAR1 in tendinopathy presumably reflects a tissue proliferative and nociceptive role. (C) 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:14471452, 2012
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