期刊
JOURNAL OF ORTHOPAEDIC RESEARCH
卷 30, 期 1, 页码 28-36出版社
WILEY-BLACKWELL
DOI: 10.1002/jor.21484
关键词
tendon; collagen; murine; transgenic; healing
类别
资金
- National Institutes of Health [AR46574-07, AR56943-01]
- National Science Foundation [0333377]
- Division Of Graduate Education
- Direct For Education and Human Resources [0333377] Funding Source: National Science Foundation
Tendon injuries are major orthopedic problems that worsen as the population ages. Type-I (Col1) and type-II (Col2) collagens play important roles in tendon midsubstance and tendon-to-bone insertion healing, respectively. Using double transgenic mice, this study aims to spatiotemporally monitor Col1 and Col2 gene expression, histology, and biomechanics up to 8 weeks following a full-length patellar tendon injury. Gene expression and histology were analyzed weekly for up to 5 weeks while mechanical properties were measured at 1, 2, 5, and 8 weeks. At week 1, the healing region displayed loose granulation tissue with little Col1 expression. Col1 expression peaked at 2 weeks, but the ECM was highly disorganized and hypercellular. By 3 weeks, Col1 expression had reduced and by 5 weeks, the ECM was generally aligned along the tendon axis. Col2 expression was not seen in the healing midsubstance or insertion at any time point. The biomechanics of the healing tissue was inadequate at all time points, achieving ultimate loads and stiffnesses of 48% and 63% of normal values by 8 weeks. Future studies will further characterize the cells within the healing midsubstance and insertion using tenogenic markers and compare these results to those of tendon cells during normal development. (c) 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:2836, 2012
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