期刊
JOURNAL OF ORGANOMETALLIC CHEMISTRY
卷 694, 期 25, 页码 4069-4075出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jorganchem.2009.08.024
关键词
6-hydroxy chromone-3-carbaldehyde thiosemicarbazone; Ni(II) complex; Crystal structure; DNA binding; Cytotoxic activity
资金
- National Natural Science Foundation of China [20475023]
A new ligand L, 6-hydroxy chromone-3-carbaldehyde thiosemicarbazone, and its Ni(II) complex have been synthesized and characterized. The crystal structure of Ni(II) complex was determined by single crystal X-ray diffraction. Ni(II) complex and ligand L were subjected to biological tests in vitro using THP-1, Raji and Hela cancer cell lines. Compared with the ligand, Ni(II) complex showed significant cytotoxic activity against these three cancer cell lines. The interactions of Ni(II) complex and ligand L with calf thymus DNA were then investigated by spectrometric titration, ethidium bromide displacement experiments and viscosity measurements methods. The experimental results indicated that Ni(II) complex bound to DNA by intercalative mode via the ligand L. The intrinsic binding constants of Ni(II) complex and ligand L with DNA were (1.10 +/- 0.65) x 10(6) M-1 and (1.48 +/- 0.57) x 10(5) M-1, respectively. (C) 2009 Elsevier B. V. All rights reserved.
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