期刊
JOURNAL OF ORGANIC CHEMISTRY
卷 79, 期 5, 页码 2308-2313出版社
AMER CHEMICAL SOC
DOI: 10.1021/jo500051y
关键词
-
资金
- Natural Sciences and Engineering Research Council (NSERC) of Canada
2'-Deoxynucleotide 5'-tetraphosphates in which a fluorescent label is attached to the terminal phosphate are used as key reagents in high-throughput DNA sequencing techniques and in single nucleotide polymorphism typing assays. We demonstrate that this class of compounds can be prepared by reacting fluorophores such as 7-hydroxy-4-methylcoumarin, methylfluorescein, fluorescein and resorufin with an activated form of cyclic trimetaphosphate to give intermediate 11. Reaction of 11 with 2'-deoxynucleoside 5'-monophosphates or a nucleoside 5'-monophosphate gave the target compounds in good yield.
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