期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 100, 期 2, 页码 757-767出版社
SPRINGER
DOI: 10.1007/s00253-015-7026-y
关键词
Alkane biosynthesis; Biofuels; Fatty acid biosynthesis; Aldehyde reductase; fadR; Escherichia coli
资金
- National Natural Science Foundation of China [31170040, 31200081]
- Chinese Academy of Sciences [KGZD-EW-606]
Concerns about energy security and global petroleum supply have made the production of renewable biofuels an industrial imperative. The ideal biofuels are n-alkanes in that they are chemically and structurally identical to the fossil fuels and can drop in to the transportation infrastructure. In this work, an Escherichia coli strain that produces n-alkanes was constructed by heterologous expression of acyl-acyl carrier protein (ACP) reductase (AAR) and aldehyde deformylating oxygenase (ADO) from Synechococcus elongatus PCC7942. The accumulation of alkanes ranged from 3.1 to 24.0 mg/L using different expressing strategies. Deletion of yqhD, an inherent aldehyde reductase in E. coli, or overexpression of fadR, an activator for fatty acid biosynthesis, exhibited a nearly twofold increase in alkane titers, respectively. Combining yqhD deletion and fadR overexpression resulted in a production titer of 255.6 mg/L in E. coli, and heptadecene was the most abundant product.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据