期刊
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
卷 67, 期 2, 页码 335-347出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.joms.2008.09.002
关键词
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资金
- NIDCR NIH HHS [R01 DE016132, R21 DE016370] Funding Source: Medline
Purpose: Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model. Materials and Methods: Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses. Results: Small tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues. Conclusions: This pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans. (C) 2009 American Association of Oral and Maxillofacial Surgeons
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