期刊
JOURNAL OF OCEANOGRAPHY
卷 64, 期 3, 页码 367-372出版社
SPRINGER
DOI: 10.1007/s10872-008-0029-x
关键词
extracellular enzyme; protease; dissolved organic matter; size fractionation; filter; adsorption
类别
In aquatic environments extracellular enzymes are bound to microbial cells or exist in a free and adsorbed state. Various filters have been used to fractionate these enzymatic activities, but enzymes may be readily adsorbed onto some materials, and such adsorption can induce errors in the estimation of enzymatic activity. In this study we examined three filters to determine the most suitable filter for fractionation when estimating proteolytic enzyme activity in seawater. We found that the polycarbonate Nuclepore membrane, widely used for size fractionation because of its pore-size accuracy, was the most favorable for this purpose, even though it adsorbed slightly more enzymes than the low-protein-binding polyethersulfone membrane. We also found that trypsin- and chymotrypsin-type enzymes were more easily adsorbed than aminopeptidases.
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