期刊
JOURNAL OF OCEANOGRAPHY
卷 64, 期 2, 页码 211-216出版社
SPRINGER
DOI: 10.1007/s10872-008-0016-2
关键词
Trichodesmium spp.; nitrogenase; whole-cell immunolocalization; immunocytochemical detection; in situ hybridization; diazotrophic cyanobacteria; horseradish peroxidase-conjugated probe; 3,3 '-diaminobenzidine
类别
The nitrogenase activities of cyanobacteria of the genus Trichodesmium are strictly light-dependent, although they do not develop heterocysts. Recently, the development of heterocyst-equivalent cells (diazocytes) was suggested in Trichodesmium spp. However, no cells with a similar appearance to diazocytes could be found in Trichodesmium sp. NIBB1067. An immunocytochemical analysis of nitrogenase in individual cells was performed using polyclonal antibodies generated from DNA fragments of genes encoding the Fe-protein and the alpha-and the beta-subunit of the MoFe-protein of mtrogenase of Trichodesmium sp. NIBB1067. Visualization of the antibody binding was carried out using a horseradish peroxidase-conjugated secondary antibody and a chromogenic substrate, 3,3'-diaminobenzidine, to avoid the masking effects caused by the bright autonuorescence emitted by Trichodesmium cells. Nitrogenase proteins were detected in almost all the cells (always higher than 95% of total cells) grown under nitrogen-limited conditions. These results indicate that Trichodesmium sp. NIBB1067 does not differentiate heterocyst-equivalent cells.
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