Intravenous Administration of Multi-walled Carbon Nanotubes Affects the Formation of Atherosclerosis in Sprague-Dawley Rats

Intravenous Administration of Multiwalled Carbon Nanotubes Affects the Formation of Atherosclerosis in Sprague-Dawley Rats: Yu-Ying Xu, et al. Zhejiang University School of Medicine, China-Background: Carbon nanotubes (CNTs) have many potential applications, including as delivery systems for a variety of diagnostic or therapeutic agents. However, it has been suggested that exposure to carbon nano-materials may be a risk for the development of vascular diseases due to its impact on the vascular endothelium. Materials and Methods: Male Sprague-Dawley rats (180-200 g) were used to generate an atherosclerosis (AS) model, and the effect of intravenous administration of multi-walled carbon nanotubes (MWCNTs) on AS was studied. To further understand the underlying mechanisms, the effects of exposure of human umbilical vascular endothelial cells (HUVECs) to MWCNTs were examined. Results: Exposure to 200 mu g/kg MWCNTs aggravated AS in this model. In addition, exposure to 50, 100 and 200 mu g/kg MWCNTs increased the calcification of the aorta in the model. Short-term exposure also revealed that 200 mu g/kg MWCNTs injured the endothelium in the aorta. MWCNTs disrupted the endothelial tight junction and induced endothelial cell death. Conclusion: The results demonstrated that MWCNTs could induce structural and functional changes in the endothelium, probably through vascular endotheliocyte injury, which eventually affected the development of AS in SD rats. (J Occup Health 2012; 54: 361-369)